![]() ![]() To test this hypothesis, we grafted the DBL or the ventral epidermis (VE) of a donor embryo into the prospective NP of a recipient embryo at stage 12/12.5 and assessed Vangl2 localization at stage 15 ( Fig. North Holland, Amsterdam, The Netherlands. Our results suggest that the dorsal blastopore lip (DBL) at the posterior end of the embryo is the source of the PCP cue. (1997) Head induction by simultaneous repression of Bmp and wnt signalling in Xenopus. Glinka, A., Wu, W., Onichtchouk, D., Blumenstock, C., and Niehrs, C. (1997) Structurally related receptors and antagonists compete for secreted wnt ligands. (1991) Overexpression of a homeodomain protein confers axis-forming activity to uncommitted Xenopus embryonic cells. (1989) Interaction between peptide growth factors and homeobox genes in the establishment of antero-posterior polarity in frog embryos. (1994) The Einsteck-method: position and structure of projections formed by implants of a ventral character. (1996) The vertebrate organizer: structure and molecules. This process is experimental and the keywords may be updated as the learning algorithm improves. These keywords were added by machine and not by the authors. However, the Einsteck can be also carried out with heterologous material, cell pellets, and injected animal caps ( 3, 4) and is more versatile than the organizer transplant. While the Einsteck is much easier to perform than the organizer transplant, the position of the implant can be less well controlled, which needs to be considered when interpreting the results ( 2). This results in the formation of an induced structure which, depending on the embryonic stage of the upper blastopore lip, is a head, trunk, or a tail. Organizer activity is revealed either by transplantation of the upper dorsal blastopore lip into the ventral side of a host gastrula or by inserting the tissue into the gastrula blastocoel (Einsteck method). Its two major inducing activities are neural induction and dorsalization of ventral mesoderm differentiation. The amphibian organizer corresponding to the upper dorsal blastopore lip of the early gastrula, is one of the classical systems in which embryonic patterning has been studied ( 1). ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |